Effects of Essential Oil Mouthwash in

Effects of Essential Oil Mouthwash in

Oral Hygiene Regimens

A 12-week study reveals exciting data on the benefits of Listerine Antiseptic

CLINICAL 2 :Effects of Essential Oil Mouthwash in Oral Hygiene Regimens


To evaluate the efficacy of various oral hygiene regimens in the prevention and reduction of plaque and gingivitis.


Single center, randomized, supervised, examiner blind, controlled clinical trial conducted at ADA-Qualified site.
  • Following a complete prophylaxis, subjects were examined for oral soft tissue status, plaque, gingivitis, and gingival bleeding at Baseline, 4 weeks, and 12 weeks.
  • If assigned to a flossing group, subjects were given flossing instructions and demonstrated competency.
  • Product use was supervised once daily virtually on weekdays with second daily and weekend product use unsupervised.



  • 213 subjects (18-60 years of age due to COVID- related health restrictions) with mild-to-severe gingivitis and no severe periodontitis, with no entry criteria on MGI/plaque, but must have a minimum of 10% gingival bleeding sites.
  • The efficacy outcomes included interproximal and whole-mouth mean modified TPI, mean MGI, gingival bleeding, bleeding on probing depth, and probing depth.


This study also demonstrated that adding Listerine® Antiseptic Cool Mint to twice-daily brushing provided statistically significant reductions in gingivitis, gingival bleeding and probing depth compared to brushing.



1. Mean Turesky Plaque Index (TPI)
**P < 0.025 superiority vs. Brushing only.
P<0.0125 superiority vs. Brush/Floss. Clinical 2 control = Brush only.


Reference: Milleman, J., Bosma, M. L., McGuire, J. A., Sunkara, A., McAdoo, K., DelSasso, A., Wills, K., & Milleman, K. (2022). Comparative Effectiveness of Toothbrushing, Flossing and Mouthrinse Regimens on Plaque and Gingivitis: A 12-week virtually supervised clinical trial. Journal of Dental Hygiene : JDH, 96(3), 21–34.

For Dental Healthcare Professionals Only. For Educational Purposes Only.